This workshop will cover the concepts of de novo assembly and initial annotation of a genome from short-read NGS data. You will be introduced to the widely-used de novo assembly pipeline, including quality checking, trimming, concepts of filtering, concepts of annotation and use a selection of tools to identify genomic features of interest.
The workshop will all be based in Genomics Virtual Lab, a platform that provides access to already installed bioinformatics tools.
Recommended Participants
Biologists and bioinformaticians interested in genome sequencing and the assembly process, particularly for bacteria. No prior bioinformatics knowledge is required.
Learning Objectives
- Perform QC evaluation and filtering on next-generation sequencing data
- Carry out a bacterial genome assembly using the Galaxy interface
- Generate preliminary annotation data for a bacterial genome sequence
- Use comparative genomics approaches to identify large genomic insertions and deletions
Syllabus
- Tools and workflows of de novo genome assembly
- Online resources for bacterial genome annotation
- Identifying genomic features of interest, including insertions, deletions and duplications
- Use of the Galaxy platform for genome analysis
| Date | Location | Registration information | ||
| 14-Jul | UQ St Lucia campus | Registration opening soon |