This hands-on practical workshop is an introduction to long read next-generation sequencing data and using SMRTLink.
Recommended Participants
Biologists and other life scientists planning to work with long-read (PacBio) next-generation sequencing data for genome assembly
Learning Objectives
- Perform QC evaluation and filtering on long read next-generation sequence data
- Use SMRTLink to assemble genome
- Visualise assembly using SMRTLink
- Assess genome quality using BUSCO and Augustus
- Export genome data files for downstream analysis
Syllabus
- Introduction to long read next-generation sequencing data
- Differences in genome assembly from short and long-read next-generation sequencing data
- Using SMRTLink to manage sample data, perform quality control filtering and for genome assembly
- Visualisation of assembled genome in SMRTLink
- Export of genome for downstream analysis and assessment of genome completeness
Date | Location | Registration information |
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